Leica tcs sp5 user manual

Conducting a zstack scan using the leica tcs sp5 confocal microscope. It is re commended setting up a separate user id for each user system administrator. Leica tcs sp5 confocal louisiana state university health. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. View online or download leica tcs sp5 x user manual, manual. This manual contains important safety directions as well as instructions for setting up the product and operating it. An acoustooptical tunable filter aotf controls the intensity of the visible excitation laser light. Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. Leica application suite x las x is the one software platform for all leica microscopes. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy.

Conducting a crosstalk test using the leica tcs sp5 confocal microscope. Two of these detectors are very sensitive hyd sp gaaspdetectors. User guide to the ibif leica tcs sp8 mp confocal microscope. Drawingalineontheannotationsheet240 drawingarectangleontheannotationsheet240. University of massachusetts medical school umass medical. This manual is the first section of a three part leica tcs sp5 user guide. National chiao tung university, bio photonics lab, bypraveen kumar.

An acoustooptical beam splitter aobs is used to split excitation. Leica tcs sp5 user manual national university of singapore. Only people who have had an introduction from kees straatman are allowed to operate the system. Log into user profile press ctrl, alt and delete simultaneously enter userid and password. The information contained in this operating manual is subject to change without prior notice. Conducting a sequential scan using the leica tcs sp5 confocal microscope. This book is both a tutorial and a reference manual for the leica tcs. If you are a new user, you should start with the chapter running the leica tcs software, use the software for a while, and then read through the rest of the book. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. Neurobiological research has a long history not only in measurements of single cells, but also of thick brain slices. The microscopy in medicine core mim core at emory university is a core facility providing access to histology and stateoftheart imaging instrumentation on a shareduse basis to faculty, staff and students of the emory division of cardiology and emory department of medicine. Tcs stands for true confocal scanner and sp5 for the 5 spectrophotometer channels this system is equipped with. Leica sp5 leica tcs sp5 user manual pdf, 166pgs sp5 short instructions pdf, 12pgs nikon a1ra1.

How to use leica confocal microscope sp5 external laser. Leica application and technology support group, life science division. You can find uptodate explanations and instructions for corresponding software functions in the las af online help. Microscopy in medicine lab emory school of medicine. This new broadband confocal microscope system perfectly merges two worlds in one. The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as well as a superz galvo stage 1500. Users may also benefit from reading what is kohler illumination. The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the leica tcs sp5 and leica tcs sp5 x. Purchase congratulations on the purchase of a leica ts16. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been. Click on qlut to display the image with false colors blue points are saturated pixels 255 level and green points are thresholded pixels 0 level. Modify zoom and image size to get the best sampling of your image see page 27 16. Purchase congratulations on the purchase of a leica ts11ts15.

New users are strongly encouraged to read the tcs sp5 user manual chapter titled introduction to confocal work which discusses many issues of confocal microscopy in a clear and direct manner. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Read carefully through the user manual before you switch on the product. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. Leica tcs sp ii comes with a context sensitive online help system that teaches you the different functions of the system.

Here more realistic results can be achieved due to the intact network. The leica tcs sp5 system covers a wide range of imaging speeds from spot recording to realtime imaging in a single truly confocal platform. Leica tcs sp5 lasaf new user guide asu core facilities. Leica tcs sp5 confocal laser excitation lasers excitation wavelength common excitable fluorophores apeak absorbance empeak emission 405 laser diode 405 nm hoechst 33258 a350 em450 acma aminochloromethoxyacridine a430 em474 dapi 4,6diamidino2phenylindole a372 em456 alexa fluor 405 a402 em421. View and download leica tcs sp5 user manual online.

The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Leica microsystems cms gmbh reserves the right to revise this. Refer to 1 safety directions for further information. Refer to 6 safety directions for further information. Leica sp5 scanning confocal cdb equipment resource. This video shows how to configure the internal laser power wavelength etc. Leica ts16 user manual optical survey equipment ltd. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. An introduction from your bosssupervisor is not enough the system has 5 detectors. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree.

All rights to this documentation are held by leica microsystems cms. In the las af software go to the configuration panel and decrease the laser power to 0%, tick off the laser check boxes. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. If you are using the argon laser, do not forget to put the digital power slider at 20 30%. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc.

Training manual leica tcs sp5 confocal microscope september 2010. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. If someone will use the microscope after you within the next 12 hours. Leica tcs sp5 confocal molecular imaging center mic. Leica tcs sp5 confocal laser scanning microscope user guide. From basic documentation to advanced life science research las x. The leica tcs sp5 is supplied with the latest version of the licensed las af software. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system. Typing your password identifies you as a valid user for this system. It is recommended setting up a separate user id for each user set up by the system administrator.

An acoustooptical beam splitter aobs is used to split excitation light and emission light instead of filter blocks. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. Read chapter las af in this user manual in order to familiarize yourself with the design and basic operation of the software. Leica microsystems sets a new standard with the integration of the leica dm6000 cfs fixed stage microscope into the leica tcs sp5 confocal platform. Move in the z direction to find your plane of interest 15. The leica sp5 confocal system has eight visible laser lines provided by a multiline argon laser, a diodepumped solid state laser, and orange and red helium neon lasers. Tune into any excitation with supercontinuum confocal. Standard operating procedures university of kentucky research. Starting the lcs software on the leica tcs sp5 confocal microscope. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Leica sp5 general training manual sept 2010 robarts. We intend this book for the novice and the experienced user. Leica tcs sp5 ii acoustooptical beam splitter products leica. Overview on general stepsoverview on general steps page 3 1.